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BLAST
Nucleotide Query
Nucleotide to Protein BLAST (blastx)
Nucleotide to Protein BLAST (blastx)
Request a New BLAST
Enter
Nucleotide
Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Or upload your own query FASTA:
Upload
The file should be a plain-text FASTA (.fasta, .fna, .fa, .fas) file. In other words, it cannot have formatting as is the case with MS Word (.doc, .docx) or Rich Text Format (.rtf). It cannot be greater than
20000MB
in size.
Don't forget to press the Upload button before attempting to submit your BLAST.
Choose Search Target
Choose from one of the
protein
BLAST databases listed below. You can also use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences.
Protein
BLAST Databases:
Select a Dataset
Or upload your own dataset:
Upload
The file should be a plain-text FASTA (.fasta, .fna, .fa) file. In other words, it cannot have formatting as is the case with MS Word (.doc, .docx) or Rich Text Format (.rtf). It cannot be greater than
20000MB
in size.
Don't forget to press the Upload button before attempting to submit your BLAST.
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
1000
5000
10000
20000
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11 Extension: 2
Existence: 10 Extension: 2
Existence: 9 Extension: 2
Existence: 8 Extension: 2
Existence: 7 Extension: 2
Existence: 6 Extension: 2
Existence: 13 Extension: 1
Existence: 12 Extension: 1
Existence: 11 Extension: 1
Existence: 10 Extension: 1
Existence: 9 Extension: 1
BLAST